Inhibition of Human Colorectal Cancer Cell Proliferation by ...

posted by:

Cancer Research

[Abstract]

Objective: To investigate the proliferation inhibitory effect of Human colorectal cancer cell line SW-480 by Genistein in vitro and to explore its antitumor molecular mechanism.Method: Human colorectal cancer cell line SW-480 was cultured in vitro and used to perform experiments. The antiproliferative effects of Genistein were examined by 3-[4,5-dimethylthiazol-2-yl]5-diphenyl tetrazolium bromid(MTT) assay in vitro.The influences of Genistein on cell growth were measured by counting cell numbers.The influences of Genistein on apoptosis and cell cycle were also detected in SW-480 cells using flow cytometry(FCM).Immunocytochemical staining were used to detect the expression of Bax? Bcl-2? NF-??? COX2.Westein bloting was used to analyze the expression of ERK, to elucidate the possible mechanism of apoptosis induced by Genistein in SW480 cells. Result:1. Genistein (of 6.25,12.5,25,50,100?mol/L ) inhibited the growth of SW-480 cells in a concentration-dependent manner.2. Flow cytometry analysis revealed that Genistein arrested cell cycle in G2/M phase and induced apoptosis in SW480 cells.3. Immunocytochemical staining and morphometric quantitative analysis indicated that down-regulation expression of bcl-2? NF-kB? COX2 protein (p

Title: Inhibition of Human Colorectal Cancer Cell Proliferation by Genistein and Its Molecular Mechanism

Category: Stomach Cancer

Filename: Inhibition of Human Colorectal Cancer Cell Proliferation by Genistein and Its Molecular Mechanism.pdf

Pages: 146

Price: US$70.00

Buy This Paper:

Download:

Donated for this research subject:

FAVORITE: ADD TO FAVORITE

Contact: E-Mail:bizdg@126.com

????????????????TEL:1-888-774-999A

How to get this paper's electronic documents?

1, Click the "Buy Now" button to complete the online payment

2, Download the paper's electronic document from the successful payment return page/Or the system will send this paper's electronic document to your E-Mail within 24 hours

Version:zh-cn

Perhaps You will be interested in these papers

Source: http://www.rescancer.com/stomach-cancer/33446.html

anna chapman kim kardashian honda generator honda generator cc sabathia ruth madoff ruth madoff